fibula
发表于 2025-3-25 05:55:22
Allele-Specific Epigenome Editingrect epigenetic editors to virtually any genomic site. This advancement in DNA-targeting technology brings allele-specific epigenome editing into reach, a “super-specific” variation of epigenome editing whose goal is an alteration of chromatin marks at only one selected allele of the target genomic
palliative-care
发表于 2025-3-25 09:58:34
Key to Delivery: The (Epi-)genome Editing Vector Toolboxpy. For this, a functional copy of the gene in question together with elements controlling its expression is produced as a vector and introduced ex vivo into the patient’s own cells that subsequently are reinfused. Alternatively, vectors are administered directly in vivo. Although this strategy resu
deforestation
发表于 2025-3-25 12:39:30
CRISPR/dCas9 Switch Systems for Temporal Transcriptional Controlase-deactivated Cas9 (dCas9) provide scientists with tools to modulate transcription of genomic loci at will by targeting transcriptional effector domains. To interrogate the temporal order of events during transcriptional regulation, rapidly inducible CRISPR/dCas9 systems provide previously unmet m
光明正大
发表于 2025-3-25 17:19:23
Delivery of Designer Epigenome Modifiers into Primary Human T Cellsies toward further understanding how gene expression is regulated and opportunities to harness these properties for therapeutic purposes. In contrast to gene knockout strategies, targeted epigenome modifications, such as editing of DNA methylation, can mediate gene expression modulation without chan
拒绝
发表于 2025-3-25 22:38:26
http://reply.papertrans.cn/32/3133/313256/313256_25.png
Aromatic
发表于 2025-3-26 00:50:35
Stable Expression of Epigenome Editors via Viral Delivery and Genomic IntegrationA-binding proteins. However, the delivery of plasmid DNA to express these fusion proteins via conventional transient transfection has certain consequences which need to be considered during the experimental design. Transient transfection achieves peak gene expression between 24 and 96 h post-transfe
火车车轮
发表于 2025-3-26 07:30:32
http://reply.papertrans.cn/32/3133/313256/313256_27.png
muffler
发表于 2025-3-26 08:32:54
Non-viral Methodology for Efficient Co-transfectionand functions of the genomic elements and epigenome modifications in an endogenous context and as new methods for treatment of diseases. Application of such technologies has drawn attention, however, to the prevailing lack of effective delivery methods. Promising viral and non-viral methods both cur
听觉
发表于 2025-3-26 14:08:26
Chromatin Immunoprecipitation in Human and Yeast Cellszation of transcription factors and posttranslational modification of histones. DNA and proteins are reversibly and covalently crosslinked using formaldehyde. Then the cells are lysed to release the chromatin. The chromatin is fragmented into smaller sizes either by micrococcal nuclease (MNase) or s
阴险
发表于 2025-3-26 20:42:47
http://reply.papertrans.cn/32/3133/313256/313256_30.png