分散
发表于 2025-3-26 23:56:51
Palgrave Macmillan Memory Studiestivity (Comoglio and Celada, 1976). In order to obivate such disadvantages, we utilized m-maleimidobenzoyl derivative of a hapten in order to couple it to sulfhydryl groups of the enzyme; a high efficiency of binding to the enzyme without appreciable reduction in enzyme activity were found.
Hallowed
发表于 2025-3-27 04:38:22
Maleimide Derivative of Hapten for Enzyme Coupling in EIAtivity (Comoglio and Celada, 1976). In order to obivate such disadvantages, we utilized m-maleimidobenzoyl derivative of a hapten in order to couple it to sulfhydryl groups of the enzyme; a high efficiency of binding to the enzyme without appreciable reduction in enzyme activity were found.
碎石头
发表于 2025-3-27 09:17:06
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fiscal
发表于 2025-3-27 13:14:30
Separation-Free Enzyme Immunoassay for Haptenslated to the analyte concentration in the sample and is monitored without separation of antibody bound and unbound fractions. This principle was later extended to a variety of enzymes and also applied to the measurement of hormones (Ullman et al., 1979) and proteins (Gibbons et al., 1980).
CAPE
发表于 2025-3-27 16:26:21
Affinity Column Mediated Immunoenzymometric Assays almost any tag ultimately capable of generating a signal, even through some complex coupling mechanism. Two fundamentally different immunometric assay configurations have been described: the one-site immunometric assay and the two site (sandwich) immunometric assay.
黄油没有
发表于 2025-3-27 20:19:10
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我不怕牺牲
发表于 2025-3-27 23:01:19
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最高峰
发表于 2025-3-28 05:19:53
lity. The great versatility of these methods, for both the identification and quantitation of clinically relevant antigens, is illustrated by example throughout this volume and is largely attributable to the stability and amplification power of enzyme labels.
友好
发表于 2025-3-28 09:41:38
https://doi.org/10.1057/9780230274044s have colorimet-rically monitored the indicating enzyme reaction. With the appropriate considerations, a fluorescence monitor can further enhance the sensitivity and selectivity of such methods without introducing significant sacrifices in the ease, speed, or cost of analysis (Kelly and Christian, 1982).
comely
发表于 2025-3-28 13:20:29
An American Worldview; An American World, i.e., sequential (noncompetitive) and competitive. The sequential, heterogenous immunoassay technique, otherwise known as the Enzyme Linked Immunosorbent Assay or ELISA, has been most widely adopted.