黑豹 发表于 2025-3-28 15:06:58
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The Physical Basis of The Direction of Timewe describe a ChIP-reChIP protocol to identify a heterotypic (asymmetric) H2A.Z–H2A-containing nucleosome. In this method, following MNase digestion of chromatin to mostly a mononucleosome fraction, H2A.Z-containing nucleosomes are first immunoprecipitated using affinity purified anti-H2A.Z antibodiAwning 发表于 2025-3-29 02:14:16
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The Physical Chemistry of MEMBRANESrch. Enhancer–promoter interactions are believed to mediate activation of target genes. Bidirectional transcription represents one hallmark of active enhancers that can be measured using transcriptome technologies such as Cap analysis of gene expression (CAGE). Recently, we have developed RNA and DNcrumble 发表于 2025-3-29 12:58:24
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Roger G. Lentle,Patrick W.M. JanssenChIP) protocol with the mass spectrometry (MS) analysis of immunoprecipitated proteins to study the combinatorial enrichment or exclusion of histone post-translational modifications (PTMs) at specific genomic regions, such as promoters or enhancers. We describe the protocol steps from the digestion能够支付 发表于 2025-3-29 20:09:03
The Physical Basis of the Direction of Timeed with gene regulation, progression through the cell cycle, and cell survival and development. Low input targeted chromatin capture (low-T2C) is an optimized version of the T2C protocol for low numbers of cells. Here, we describe the protocol for low-T2C, including all experimental steps and bioinformatics tools in detail.Progesterone 发表于 2025-3-30 01:20:45
Low Input Targeted Chromatin Capture (Low-T2C)ed with gene regulation, progression through the cell cycle, and cell survival and development. Low input targeted chromatin capture (low-T2C) is an optimized version of the T2C protocol for low numbers of cells. Here, we describe the protocol for low-T2C, including all experimental steps and bioinformatics tools in detail.Receive 发表于 2025-3-30 07:15:26
Tilman Borggrefe,Benedetto Daniele GiaimoIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts