Pruritus
发表于 2025-3-23 11:13:07
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旋转一周
发表于 2025-3-23 15:16:07
Important Assay Details,Conducting an Elispot assay is a multistep process. The main steps are:
guardianship
发表于 2025-3-23 21:42:33
Reagents and Materials,The choice of the Elispot plate can dramatically influence the final results of an Elispot experiment. Membrane-based 96-well Elispot plates are the optimal choice for Elispot. The membrane fibers look like a tightly woven web of polymers that offers a large surface for protein binding (Fig. 3.1).
omnibus
发表于 2025-3-24 01:47:28
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六个才偏离
发表于 2025-3-24 03:12:53
Planning the Experiment,A good Elispot experiment starts with a well-thought-through plan of execution. The quality of an experiment and its results are determined by the controls it includes. Here is a summary of important controls:
transient-pain
发表于 2025-3-24 09:59:53
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superfluous
发表于 2025-3-24 11:16:41
Quality Control,Throughout the book we have touched upon various important controls, for the sample (apoptosis and viability, see Sect. 4.6), and for the assay (negative controls, sample-specific internal controls, nonspecific positive controls, external controls, see Sect. 5.1).
Infiltrate
发表于 2025-3-24 14:57:06
Troubleshooting,This book has been structured in a way that it provides detailed information on choices about how to run an optimized Elispot assay. Throughout the book examples are given for what works and what does not and for good and suboptimal outcomes, and recommendations on how to prevent suboptimal outcomes and the reasoning behind it are provided.
Neolithic
发表于 2025-3-24 22:40:13
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Blemish
发表于 2025-3-25 02:25:35
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