奇怪 发表于 2025-3-28 15:28:56
https://doi.org/10.1007/978-1-4614-1581-7 pores in the cell membrane. This is done by applying an electrical field to a suspension of cells with the transfectant (e.g., drugs) in a container with electrodes. Applications include preparation of gene libraries and genetic manipulation. Recently, however, the technology has gone beyond researConfidential 发表于 2025-3-28 18:46:48
Guide to Effective Grant Writingbrane, facilitating uptake of plasmid DNA that can become expressed transiently and, eventually, be stably incorporated into the genome..The major electrical parameters are field strength and pulse duration, which are inversely related and can be chosen within wide ranges (100–5000 V/cm and 0.01–100Heterodoxy 发表于 2025-3-29 02:41:29
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https://doi.org/10.1007/978-1-84882-543-7t-derived cells, and shoot regeneration from protoplast-derived tissues. Such effects of electrostimulation persist over many cell generations. This enhancement of growth and organogenesis has application in the multiplication of elite individuals and in maximizing the recovery of genetically engine宫殿般 发表于 2025-3-29 11:44:28
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https://doi.org/10.1007/978-3-319-32794-5osmotic pressure of the cytoplasmic fluid and the extracellular medium. Experiments show that electrical stimulation introduces pores of limited sizes in the plasma membrane. These pores can be resealed without losing the cytoplasmic macromolecular contents, and most cells will survive after pore remastopexy 发表于 2025-3-30 06:54:38
Alexis Kirke,Eduardo R. Miranda in the fusion process. The results obtained thus far suggest several potential applications of cell-tissue electrofusion, including site-specific delivery systems, improved surgical procedures, and the establishment of unique animal models.