免除责任 发表于 2025-3-25 07:00:58
Navin C. Nanda,Joel S. Raichlention of tagged and particularly fluorescent or luminescent viruses can be applied as tools for both diagnostic applications and for screening to identify novel countermeasures. Here we describe the generation and basic characterization of recombinant Ebola viruses rescued from cloned cDNA using a T7-driven system.实现 发表于 2025-3-25 08:29:37
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Forty Years of Ebolavirus Molecular Biology: Understanding a Novel Disease Agent Through the Developh, if not driven by, advancements in the technical methodologies available for these kinds of studies. Here we review of the current state of our knowledge regarding ebolavirus biology and emphasize the associated methods that made these discoveries possible.吼叫 发表于 2025-3-25 19:18:47
Nonradioactive Northern Blot Analysis to Detect Ebola Virus Minigenomic mRNArect study of .-acting regulatory regions as well as .-acting factors involved in Ebola virus minigenome transcription compared to the indirect measurement of reporter protein activity that additionally reflects translational effects (. Chapter . in this book for details).宣誓书 发表于 2025-3-25 23:23:49
Generation of Recombinant Ebola Viruses Using Reverse Geneticstion of tagged and particularly fluorescent or luminescent viruses can be applied as tools for both diagnostic applications and for screening to identify novel countermeasures. Here we describe the generation and basic characterization of recombinant Ebola viruses rescued from cloned cDNA using a T7-driven system.加花粗鄙人 发表于 2025-3-26 02:52:22
Luciferase-Expressing Ebolaviruses as Tools for Screening of Antivirals short assay times. Here we provide a detailed protocol for the use of such a luciferase-expressing reporter virus for antiviral screening in a 96-well format, with parallel assessment of cytotoxicity of the screened compounds.Minutes 发表于 2025-3-26 05:20:58
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http://reply.papertrans.cn/31/3011/301062/301062_29.pngReservation 发表于 2025-3-26 19:42:12
A Semi-automated High-Throughput Microtitration Assay for Filovirusesng a luminescent-based cell viability assay and an automated plate reader, has improved workflow efficiency, reduced operator burden and assay time, decreased assay variability, and increased data return.