Harpoon
发表于 2025-3-25 08:29:23
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MELD
发表于 2025-3-25 14:00:36
Measuring ERK Activity Dynamics in Single Living Cells Using FRET Biosensors,dynamic datasets. In this chapter, we describe the analysis of endogenous extracellular signal-regulated kinase (ERK) activity in living cells using the EKAR2G (ERK activity reporter second generation) probe. We focus on the generation of stable cell lines expressing the EKAR2G sensor as well as data acquisition and analysis.
假装是我
发表于 2025-3-25 19:42:40
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acetylcholine
发表于 2025-3-25 21:18:04
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完成才能战胜
发表于 2025-3-26 01:56:27
https://doi.org/10.1007/978-3-322-80760-1nt isolation of native ERK signaling complexes, which are suitable for subsequent analysis by mass spectrometry. Our analysis of the ERK interactome has identified both known and novel signaling components. This method can be easily adapted for SBP-based purification of protein complexes in any expression system.
自传
发表于 2025-3-26 06:24:25
https://doi.org/10.1007/978-3-476-99011-2p between actin tension and ERK phosphorylation is missing. In this chapter, we describe our novel methods to quantify tensile force and ERK phosphorylation on individual actin stress fibers. These methods have enabled us to show that ERK is activated on stress fibers in a tensile force-dependent manner.
modish
发表于 2025-3-26 10:05:42
https://doi.org/10.1007/978-3-642-51143-1newal and early lineage commitment. In this chapter, we describe methods used for the isolation and establishment of mouse ES cell lines from blastocyst embryos and for the measurement of ERK1/2 activity in ES cells.
遵循的规范
发表于 2025-3-26 16:14:18
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Vital-Signs
发表于 2025-3-26 16:57:47
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ABYSS
发表于 2025-3-27 00:01:12
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