TEMPO 发表于 2025-3-21 17:18:05

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嫌恶 发表于 2025-3-21 22:59:09

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fetter 发表于 2025-3-22 02:28:17

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独行者 发表于 2025-3-22 04:42:08

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漂白 发表于 2025-3-22 11:12:24

Extraction of DNA from Agarose Gels, elution of the DNA from the substrate. Other methods include hot phenol extraction of the DNA from the gel. The use of phenol is best avoided if possible because of safety and waste disposal concerns. Presented here are two alternative techniques that avoid these concerns, do not require the purcha

有特色 发表于 2025-3-22 12:53:23

,Construction of Genomic Libraries in λ-Vectors,cloning sites facilitating the cloning of foreign DNA. In addition, λ-phage vectors have the following advantageous features: high cloning efficiency, a relatively large insert-size capacity, and suitability for screening using nucleic acid probes. In terms of gene screening, genomic DNA libraries a

有特色 发表于 2025-3-22 17:58:20

Isolation of Plasmids from , by Boiling Lysis,described with different adaptations in a variety of protocol books (.,.). The quality of the isolated plasmid DNA is lower than that from an alkaline lysis miniprep, but it is sufficient for restriction analysis.

Judicious 发表于 2025-3-23 01:00:16

Cloning in Plasmid Vectors,d upon transformation of the recombinant molecule into a bacterial cell (.. and .) so that the DNA of interest can be investigated further. Cloning is an essential part of many experiments, including library generation (..) and expression studies (..).

美学 发表于 2025-3-23 02:05:43

E. coli Plasmid Vectors978-1-59259-409-2Series ISSN 1064-3745 Series E-ISSN 1940-6029

河潭 发表于 2025-3-23 05:33:46

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查看完整版本: Titlebook: E. coli Plasmid Vectors; Methods and Applicat Nicola Casali,Andrew Preston Book 20031st edition Springer Science+Business Media New York 20