种类
发表于 2025-3-25 04:17:41
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ungainly
发表于 2025-3-25 08:08:16
https://doi.org/10.1007/978-3-642-57192-3es. We provide updated and optimized protocols for the expression and purification of a tail-truncated single-headed . dynein, for polarity-marked MT polymerization, and for the non-covalent attachment of MTs to cover glass surfaces for the measurement of dynein-MT detachment forces.
fatty-streak
发表于 2025-3-25 15:08:29
Employing Live-Cell Imaging to Study Motor-Mediated Transport users to examine the net effect of motors and motor-regulatory proteins on organelle distribution. Together, these methods open the door to motility experiments that more fully interrogate the complex cellular environment.
百灵鸟
发表于 2025-3-25 17:13:26
Measurements of the Force-Dependent Detachment Rates of Cytoplasmic Dynein from Microtubuleses. We provide updated and optimized protocols for the expression and purification of a tail-truncated single-headed . dynein, for polarity-marked MT polymerization, and for the non-covalent attachment of MTs to cover glass surfaces for the measurement of dynein-MT detachment forces.
宠爱
发表于 2025-3-25 22:40:41
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改正
发表于 2025-3-26 02:37:39
Using Optogenetics to Spatially Control Cortical Dynein Activity in Mitotic Human Cellstion and manipulate downstream events. In this chapter, I will present methods for locally activating cytoplasmic dynein at the mitotic cell cortex in human cells, with a focus on how to generate knock-in cell lines and set up a microscope system.
GRE
发表于 2025-3-26 06:44:13
Reconstitution of Dynein/Dynactin Transport Using Recombinant Dynein adaptors, and endogenous dynactin and detail a protocol for the single-molecule motility assay. In this assay, microtubules are first immobilized on a coverslip. A fluorescently labeled dynein/dynactin/cargo adaptor complex is then added, allowing for the measurement of key motility parameters as the complex walks along the microtubule.
hermetic
发表于 2025-3-26 10:25:13
Fast and Easy Transient Mammalian Cell Expression and Purification of Cytoplasmic Dyneinpurification for high-throughput protein engineering. Mammalian cell expression might be generally considered to be a laborious process, but with recent technology and some simple inexpensive custom-built labware, dynein expression and purification from mammalian cells can be fast and easy.
achlorhydria
发表于 2025-3-26 16:25:41
https://doi.org/10.1007/978-3-642-57192-3external factors affect the microtubule-detachment rate and velocity of dynein. We also briefly discuss the remaining challenges and future directions of optical trapping studies of dyneins and other microtubule-based motors.
胡言乱语
发表于 2025-3-26 19:33:51
Studying Dynein Mechanochemistry with an Optical Trapexternal factors affect the microtubule-detachment rate and velocity of dynein. We also briefly discuss the remaining challenges and future directions of optical trapping studies of dyneins and other microtubule-based motors.