ALTER
发表于 2025-3-30 11:17:31
https://doi.org/10.1007/978-3-642-71028-5to introduce circular permutation. Our RF cloning method made the protocol faster and more cost-effective. In this chapter, we describe a step-by-step protocol for generating circular permutants using RF methodology.
panorama
发表于 2025-3-30 15:05:23
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解开
发表于 2025-3-30 20:20:03
SpeedyGenesXL: an Automated, High-Throughput Platform for the Preparation of Bespoke Ultralarge Varbinatorial-OR-type libraries based on the SpeedyGenes methodology. Together this offers a flexible platform for library synthesis, capable of generating many different bespoke, diverse libraries simultaneously.
MELD
发表于 2025-3-30 22:41:45
Facile Assembly of Combinatorial Mutagenesis Libraries Using Nicking Mutagenesis, 14 mutated positions, a total of 16,384 library variants, and a protocol for the generation of large, user-defined combinatorial libraries. The reader can use this protocol to create such libraries in 2 days.
智力高
发表于 2025-3-31 01:17:38
GeneORator: An Efficient Method for the Systematic Mutagenesis of Entire Genes,mental workload. The method describes library synthesis using asymmetric PCR, in which mutagenic primers are designed to create OR-type mutations at multiple sites of variation in a two-step protocol. As an example, we show how this can be utilized for controlled and economical mutagenesis of every amino acid codon in a gene.
我要沮丧
发表于 2025-3-31 05:21:52
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怎样才咆哮
发表于 2025-3-31 09:17:11
Application of Restriction Free (RF) Cloning in Circular Permutation,to introduce circular permutation. Our RF cloning method made the protocol faster and more cost-effective. In this chapter, we describe a step-by-step protocol for generating circular permutants using RF methodology.