总 发表于 2025-3-25 06:11:06
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Müfit Bahadir,Harun Parlar,Michael Spiteller,.). The first step is construction of a mutant library, usually accomplished by random point mutagenesis with error-prone PCR (.) or by DNA shuffling (recombination) (.,.). The second, and most critical step is finding the desired mutants by screening or selection of the libraries. Screens for enzynerve-sparing 发表于 2025-3-25 19:32:32
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Springer-Handbuch der Mathematik Ie of its relative ease and high throughput compared to liquid-phase screening in multi-well plates. As with any high throughput screening approach, a suitable colorimetric or fluorimetric assay must exist (or be developed) for the enzyme function in question. There are two major requirements that mu痛恨 发表于 2025-3-26 01:40:37
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Numerik und Wissenschaftliches Rechnen,(.). They contain a noncovalently bound protoporphyrin IX, giving these enzymes characteristic spectral properties. This heme has an available sixth coordination ligand that is able to bind carbon monoxide. Difference spectroscopy yields a spectral peak at approx 450 nm when comparing bound and unbocrockery 发表于 2025-3-26 15:30:46
Planung, Entscheidung und Kontrolle,l aromatic oxidation reactions are prone to byproduct formation and often require heavy-metal catalysts, extremes of temperature and pressure, and explosive reagents (.). In contrast, biocatalysts such as mono- and dioxygenases perform the same chemistry in water at ambient conditions, usually withhurricane 发表于 2025-3-26 17:06:37
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