悄悄移动
发表于 2025-3-28 16:51:10
be highly effective in identifying sequences that are differentially expressed in various cell types. The most striking advantage is, however, that only nanograms of total RNA are sufficient. Thus every mRNA species expressed in the cell system can be investigated, even those present at very low levels.978-3-540-63297-9978-3-642-80454-0
Camouflage
发表于 2025-3-28 19:18:57
Quantities, laws and sign conventions a different ddNTP, are performed. When a radionucleotide is included in the reaction, the resulting labeled fragments, with the same origin but ending at a different nucleotide, are visualized by autoradiography after separation by denaturing gel electrophoresis.
intellect
发表于 2025-3-29 01:34:47
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四海为家的人
发表于 2025-3-29 04:49:41
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外观
发表于 2025-3-29 09:34:07
Size Separation of cDNA Fragments,denaturing ones, since both incompletely annealed single-stranded cDNAs and heteroduplexes (produced by errors of Taq polymerase) from the same gene may still be resolved as different bands by nondenaturing gel.
扔掉掐死你
发表于 2025-3-29 13:04:29
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Infect
发表于 2025-3-29 17:36:38
Preference over Worlds: Static Logics, may be used. However, none of them ensure 100 % reliability for accurate cutting of specific bands from the gel. We suggest following the procedure described by Kim et al. (1995) and reported below. This technique is more reliable than others, since guidelines exist on how to overlap the gel and the X-ray film.