撤退 发表于 2025-3-25 05:57:15
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https://doi.org/10.1007/978-94-009-8808-8olving sample preparation, protein extraction, differential fluorescence labeling using a 3-dye system, first-dimension isoelectric focusing, second-dimension slab gel electrophoresis, DIGE image analysis, protein digestion, and mass spectrometry.conjunctivitis 发表于 2025-3-25 15:09:31
Two-Dimensional Gel Electrophoresis and 2D-DIGEthe principles of 2D-PAGE and 2D-DIGE including limitations to the methods. 2D-PAGE and 2D-DIGE continue to be popular methods in bioprocessing-related research (particularly on recombinant Chinese hamster ovary cells), which will also be discussed in the review chapter.否决 发表于 2025-3-25 16:28:15
Comparative Two-Dimensional Fluorescence Gel Electrophoresisl control of the x-dimension (p.), azo dyes can be used. Experiments are possible in both vertical and horizontal (h) electrophoresis devices, but hCoFGE is much easier to perform. For data analysis, commercial software capable of warping can be adapted.exacerbate 发表于 2025-3-25 22:52:47
Comparative 3-Sample DIGE Analysis of Skeletal Musclesolving sample preparation, protein extraction, differential fluorescence labeling using a 3-dye system, first-dimension isoelectric focusing, second-dimension slab gel electrophoresis, DIGE image analysis, protein digestion, and mass spectrometry.掺假 发表于 2025-3-26 02:44:42
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Comparative Testis Tissue Proteomics Using 2-Dye Versus 3-Dye DIGE Analysis of tissue protein compositions. Comparing tissue groups under different conditions is crucially important for advancing the biomedical field by characterization of cellular processes, understanding pathophysiological development and tissue biomarker discovery. This chapter discusses 2D-DIGE as a co