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Müfit Bahadir,Harun Parlar,Michael Spitelleron technique in yeast genetics known as “plasmid shuffling,” where the wild-type copy of the Pol3 present in a Ura3 selective marker plasmid is exchanged or genetically complemented for in vitro mutated version(s) of Pol3 in the domain-of-interest. Since Pol3p is essential for viability of yeast, on