REIGN
发表于 2025-3-28 15:10:53
Identification of Deubiquitinase Substrates in , by Systematic Overexpression, in the cell, and second, redundancy between DUBs complicates loss of function screening approaches. Here, we describe a systematic overexpression approach that takes advantage of genome-wide resources available in . to overcome these challenges and identify DUB substrates in cells.
wangle
发表于 2025-3-28 19:41:54
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假
发表于 2025-3-29 01:20:08
Amit Patel,Andrew Crooks,Naoru Koizumilso outline the application of archaea in forming Ub/Ubl isopeptide linkages that include the covalent attachments of diverse archaeal and eukaryotic Ub/Ubls to target proteins. Archaea form these Ub/Ubl-linked protein conjugates in vivo, and the resulting products are found to serve as useful DUB substrates for in vitro assays.
CRATE
发表于 2025-3-29 06:25:25
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污秽
发表于 2025-3-29 10:17:50
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CALL
发表于 2025-3-29 14:29:12
https://doi.org/10.1007/978-3-030-21406-7e pinpoint DUBs sufficient to counteract proteasomal degradation of these newly identified substrates. We apply this method to . egg extract but suggest that it can also be adapted to other cell lysates.
Ataxia
发表于 2025-3-29 17:30:18
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中国纪念碑
发表于 2025-3-29 22:41:20
Identification of Deubiquitinase Substrates in , Egg Extract,e pinpoint DUBs sufficient to counteract proteasomal degradation of these newly identified substrates. We apply this method to . egg extract but suggest that it can also be adapted to other cell lysates.
谎言
发表于 2025-3-30 00:52:44
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Nostalgia
发表于 2025-3-30 04:47:08
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