折磨
发表于 2025-3-28 15:11:09
Isolation and Titration of Dengue Viruses by the Mosquito Inoculation Techniquequitoes. It has been under utilized since it was described 40 years ago because most dengue laboratories do not have access to an insectary to rear mosquitoes. This technique requires good eye-hand coordination while doing manipulation under a stereoscopic microscope, and extensive practice is neede
充满人
发表于 2025-3-28 19:41:27
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馆长
发表于 2025-3-29 01:05:22
Dengue Virus Purification and Sample Preparation for Cryo-Electron Microscopy During the last few decades, significant progress has been made where virus structures previously resolved only to low resolution have now breached the sub-nanometer threshold. Critical to such excellent results are the acquisition of highly purified virus samples and well-frozen samples in vitreou
愤慨一下
发表于 2025-3-29 04:59:32
Development of a Multiplex Bead-Based Assay to Monitor Dengue Virus Seroconversionever, the presence of envelope-specific antibodies against a given serotype may contribute to enhanced disease during secondary infection with another serotype. There is a need therefore for a standardized, high-throughput low-volume assay which permits the simultaneous screening of reactivity to mu
defeatist
发表于 2025-3-29 08:26:55
Pseudo-infectious Reporter Virus Particles for Measuring Antibody-Mediated Neutralization and Enhancmediated neutralization and enhancement of DENV infection. RVPs are pseudo-infectious virions produced by complementation of a self-replicating flavivirus replicon with the DENV structural genes in trans. RVPs harvested from transfected cells are capable of only a single round of infection and encap
FRAUD
发表于 2025-3-29 15:17:06
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不如屎壳郎
发表于 2025-3-29 17:14:50
Development and Application of Dengue Virus Reverse Genetic Systems technological platform for advancing dengue virus research. Mutations can be introduced into the genome to study their effect on virus replication and pathogenesis while attenuated or chimeric viruses can be constructed that are potential vaccine candidates. The deletion of the virus structural gen
Lice692
发表于 2025-3-29 21:01:44
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分开如此和谐
发表于 2025-3-30 00:31:51
Targeted Mutagenesis of Dengue Virus Type 2 Replicon RNA by Yeast In Vivo Recombination gives important clues to their function in the viral life cycle. However, a major challenge to the establishment of a reverse genetics system for flaviviruses is the instability of their nucleotide sequences in .. Thus, direct cloning using conventional restriction enzyme-based procedures usually l
现代
发表于 2025-3-30 06:34:36
Identification of Dengue-Specific Human Antibody Fragments Using Phage Displayepertoires displayed on M13 filamentous bacteriophage is described. Naïve repertoires are unbiased, thus enabling the identification of antibodies to dengue structural and nonstructural proteins from the same library. Dengue-specific clones are enriched by binding to an immobilized dengue antigen, f