synchronous 发表于 2025-3-30 11:52:40
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Eugene Valkov,Aaron C. GoldstrohmIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the expertsVEN 发表于 2025-3-30 21:42:27
Methods in Molecular Biologyhttp://image.papertrans.cn/d/image/263953.jpgComedienne 发表于 2025-3-31 03:24:57
Reconstitution of Human CCR4-NOT Complex from Purified Proteins and an Assay of Its Deadenylation Ainfected insect cells, purified using column chromatography, and reconstituted into a stable complex containing all eight core subunits. In addition, we describe the biochemical assay of deadenylation using the reconstituted complex.物种起源 发表于 2025-3-31 07:51:58
Transcriptome-Wide Analysis of mRNA Adenylation Status in Yeast Using Nanopore Sequencing,ics. However, direct RNA sequencing using the Oxford Nanopore Technologies (ONT) platform makes it possible to conduct transcriptome-wide analyses at the single-molecule level without the PCR bias introduced by other methods. In this chapter, we provide a protocol to measure both RNA levels and poly(A)-tail lengths in the yeast . using ONT.演绎 发表于 2025-3-31 11:13:01
Reconstitution of Human CCR4-NOT Complex from Purified Proteins and an Assay of Its Deadenylation Ainfected insect cells, purified using column chromatography, and reconstituted into a stable complex containing all eight core subunits. In addition, we describe the biochemical assay of deadenylation using the reconstituted complex.百灵鸟 发表于 2025-3-31 15:11:39
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http://reply.papertrans.cn/27/2640/263953/263953_59.png–FER 发表于 2025-3-31 23:10:33
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