Hectic 发表于 2025-3-25 03:31:02
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Ethylation Interference,s between macromolecules that juxtapose the specific recognition elements. As part of these processes, proteins make a large number of contacts to the phosphodiester backbone of DNA, as was predicted from biochemical assays of the ionic strength dependence of DNA binding.发起 发表于 2025-3-25 14:07:42
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Martina Lucht,Steffi Domagk,Martin Mohringy useful for qualitative purposes, EMSA has the added advantage of being suitable for quantitative and kinetic analyzes (.). Furthermore, because of its very high sensitivity, EMSA makes it possible to resolve complexes of different protein or DNA stoichiometry (.) and even to detect conformational changes.STENT 发表于 2025-3-25 20:50:16
https://doi.org/10.1007/978-3-031-52407-3egative superhelicity in free DNA. Both kinks and localized unwinding can arise transiently during the enzymatic manipulation of DNA by recombinases and by protein complexes involved in the establishment of unwound regions during the initiation of transcription or of DNA replication.macabre 发表于 2025-3-26 02:40:40
https://doi.org/10.1007/978-981-16-0771-4teraction. Furthermore, in the presence of a large number of tryptophan and tyrosine residues, a relatively small perturbation in the overall emission spectrum brought about by DNA binding may be masked.white-matter 发表于 2025-3-26 06:04:52
Electrophoretic Mobility Shift Assays for the Analysis of DNA-Protein Interactions,y useful for qualitative purposes, EMSA has the added advantage of being suitable for quantitative and kinetic analyzes (.). Furthermore, because of its very high sensitivity, EMSA makes it possible to resolve complexes of different protein or DNA stoichiometry (.) and even to detect conformational changes.恶名声 发表于 2025-3-26 10:26:16
Diffusible Singlet Oxygen as a Probe of DNA Deformation,egative superhelicity in free DNA. Both kinks and localized unwinding can arise transiently during the enzymatic manipulation of DNA by recombinases and by protein complexes involved in the establishment of unwound regions during the initiation of transcription or of DNA replication.撕裂皮肉 发表于 2025-3-26 15:46:00
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Stefania Montani,Giorgio Leonardictions (.), before being introduced to DNA-protein interaction studies by Jones and Berg in 1966 (.). The principle of the technique is straightforward. Under a wide range of buffer conditions, nucleic acids pass freely through membrane filters, whereas proteins and their bound ligands are retained.