协奏曲
发表于 2025-3-25 05:58:54
Lecture Notes in Computer Scienceusing bulky nucleases such as DNase I. The DNase I footprinting method was developed to take advantage of this fact in the study of DNA-protein interactions: it consists in comparing the pattern of fragments generated by the partial digestion of a DNA sequence in the absence of a protein to that pro
habile
发表于 2025-3-25 10:02:33
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Lipoma
发表于 2025-3-25 15:22:12
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airborne
发表于 2025-3-25 18:19:22
Mar Marcos,Jose M. Juarez,Gregor Stiglicled DNA with a gel blot, washing, and visualizing through autoradiography. A blot resulting from 1-dimensional SDS-PAGE reveals the molecular weight of the binding proteins. To increase separation and determine isoelectric point a 2-dimensional gel can be blotted. Additional dimensions of electropho
Coronation
发表于 2025-3-25 23:36:00
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倒转
发表于 2025-3-26 01:50:49
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可以任性
发表于 2025-3-26 07:18:10
Olga Dolinina,Igor Bessmertny,Vadim Zhmud and repair. Identifying and examining the nature of these interactions is therefore a crucial prerequisite to understand the molecular basis of how these fundamental processes take place. The application of fluorescence techniques and in particular fluorescence resonance energy transfer (FRET) to p
interior
发表于 2025-3-26 08:57:30
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显而易见
发表于 2025-3-26 13:58:50
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LASH
发表于 2025-3-26 17:36:42
https://doi.org/10.1007/978-3-031-06242-1y only a weak sequence preference, making the analysis of their DNA-binding characteristics difficult if not impossible in a standard electrophoretic mobility shift assay (EMSA). In contrast, such proteins often bind prebent DNAs with high affinity and specificity. A synthetic cruciform DNA structur