Memorial
发表于 2025-3-23 12:43:20
Methods That Make Your Cloning Life Easier,In a modern biology lab, subcloning a gene obtained by RT-PCR into an expression vector and then expressing it in mammalian cells or . are routinely performed. Sometimes, a cloned gene is directly inserted into an expression vector. Other times, it is inserted into a small vector with MCS and then transferred to an expression vector.
reaching
发表于 2025-3-23 14:03:04
Advice to Cloners,Cloning failures keep me awake at night, especially when what appears to be easy-looking cloning did not work out.
Organization
发表于 2025-3-23 18:27:46
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Prophylaxis
发表于 2025-3-24 02:06:28
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漂亮
发表于 2025-3-24 05:19:28
Seok-Yong Choi,Hyunju Ro,Hankuil YiClear step-by-step description of the full range of methods.Advice on fundamentals and prerequisites.Guidance on avoidance of errors
阻止
发表于 2025-3-24 09:01:00
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性行为放纵者
发表于 2025-3-24 13:03:08
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–FER
发表于 2025-3-24 17:18:16
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原来
发表于 2025-3-24 22:18:01
The Last Steps of Cloning,u enter the name of a gene into UniGene, for example Gene X, you get orthologs found in various organisms. Of course, this search is limited to information on Gene X and its orthologs catalogued in UniGene. In recent years, the gene data in UniGene have been accumulating exponentially.
hardheaded
发表于 2025-3-24 23:59:53
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