闪光你我
发表于 2025-3-30 10:37:50
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拔出
发表于 2025-3-30 16:23:18
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果仁
发表于 2025-3-30 16:39:40
Topological and Algebraic Considerationsdouble-stranded DNA probe containing the sequence recognized by the factor in question. Recombinants expressing a protein capable of binding the probe sequence in the presence of nonspecific competitor DNA are thus identified and can be isolated.
否决
发表于 2025-3-31 00:39:38
Book 1997iptase) to convert poly A* mRNA into double-stranded cDNA suitable for insertion into prokaryotic vectors. Since then cDNA technology has become a fundamental tool for the molecular biologist and at the same time some very significant advances have occurred in the methods for con structing and scre
hair-bulb
发表于 2025-3-31 01:44:58
Applications in Statistical ComputingA fragments, In this case, treatment of the mRNA with a denaturant, such as methyl-mercuric hydroxide, prior to synthesis may be necessary (.). Other potential difficulties include DNA molecules contaminating the mRNA sample. DNA can clone efficiently, and their introns can confuse results. RNase-free DNase treatment of the sample is recommended.
不能逃避
发表于 2025-3-31 05:55:04
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Cardioversion
发表于 2025-3-31 10:05:09
Pascal Kerschke,Heike Trautmannnd, the intrinsic difficulty of multiple sequential enzymatic reactions required for cDNA cloning often leads to low yields and truncated clones (.). Finally, screening of a library with hybridization technique is time consuming.
争吵加
发表于 2025-3-31 17:14:44
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Aids209
发表于 2025-3-31 18:43:48
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degradation
发表于 2025-3-31 22:13:01
,cDNA Library Construction for the Lambda ZAP®-Based Vectors,A fragments, In this case, treatment of the mRNA with a denaturant, such as methyl-mercuric hydroxide, prior to synthesis may be necessary (.). Other potential difficulties include DNA molecules contaminating the mRNA sample. DNA can clone efficiently, and their introns can confuse results. RNase-free DNase treatment of the sample is recommended.