不幸的人
发表于 2025-3-26 23:59:36
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Presbycusis
发表于 2025-3-27 01:21:17
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instructive
发表于 2025-3-27 07:46:29
Imaging the cAMP Signaling Microdomain of the Primary Cilium Using Targeted FRET-Based Biosensors,lls using targeted genetically encoded FRET biosensors. Key experimental difficulties include gathering sufficient signal from such a small, photon-limited volume, and the susceptibility of cilia to movement artifacts. Other challenges are associated with the fidelity of sensor targeting and the dif
macabre
发表于 2025-3-27 10:54:17
,Disruptors of AKAP-Dependent Protein–Protein Interactions,n interactions. In part, this is due to the lack of agents for specifically targeting defined protein–protein interactions. Peptidic and non-peptidic inhibitors are invaluable molecular tools for elucidating the functions of AKAP-dependent protein–protein interactions. In addition, such interaction
无节奏
发表于 2025-3-27 14:16:58
Micro-2D Cell Culture for cAMP Measurements Using FRET Reporters in Human iPSC-Derived Cardiomyocyt FRET measurements with this sensor and the analysis of generated imaging data. Numbers for seeding areas, seeding densities, coating volumes and concentrations, media volumes, and concentrations of reagents are given as guidelines.
anagen
发表于 2025-3-27 20:44:42
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闹剧
发表于 2025-3-28 00:57:32
How to Make the CUTiest Sensor in Three Simple Steps for Computational Pedestrians,60 nM)..This simple protocol, which integrates our previous experience, only requires free web servers and can be straightforwardly used to create cAMP sensors adapted to the physicochemical characteristics of known cyclic nucleotide-binding domains.
Flirtatious
发表于 2025-3-28 04:32:50
Biochemical Analysis of AKAP-Anchored PKA Signaling Complexes,. The latter approach evaluates the composition of PKA holoenzymes, in which regulatory subunits and catalytic subunits can be visualized in the presence of test compounds and small-molecule inhibitors.
misanthrope
发表于 2025-3-28 10:12:45
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FLAG
发表于 2025-3-28 11:40:30
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