Allergic
发表于 2025-3-30 09:39:17
https://doi.org/10.1007/978-1-4939-2537-7Adenosine 3′,5′-monophosphate; Cellular function; Disease mechanisms; Protein kinase A (PKA); Signaling
亲属
发表于 2025-3-30 13:48:07
978-1-4939-4950-2Springer Science+Business Media New York 2015
与野兽博斗者
发表于 2025-3-30 18:23:29
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幼儿
发表于 2025-3-31 00:41:49
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拘留
发表于 2025-3-31 04:41:13
https://doi.org/10.1007/978-3-319-63321-3A), can be measured in living cells, thanks to genetically encoded probes based on fluorescence resonance energy transfer (FRET). While these biosensors enable the assessment of cAMP or PKA activity with great spatial and temporal resolution, concomitant events triggered by the same stimuli at the s
ovation
发表于 2025-3-31 05:54:15
Bernardo Cardoso,Jorge Ferraz de Abreuwnstream cellular effectors. FRET-based sensors are ideal to visualize and measure these rapid changes of second messenger concentrations in time and place. Here, we describe the use of EPAC-based FRET sensors to measure cAMP with spatiotemporal resolution in cells by fluorescence lifetime imaging (
fibula
发表于 2025-3-31 12:21:32
Applications and Usability of Interactive TVcAMP) signaling and compartmentalization in living cells. These sensors allow estimation of relative changes of cAMP levels in real-time and intact cells. However, one of their major shortcomings is that they do not easily allow direct measurement of cAMP concentrations. This is mainly due to the fa
改变
发表于 2025-3-31 14:31:51
Applications and Usability of Interactive TV of local concentrations of second messengers in living cells. Nowadays, the availability of a number of 3D structures of cyclic nucleotide-binding domains (CNBD) undergoing conformational transitions upon cAMP binding, along with computational tools, can be exploited for the design of novel or impr
TERRA
发表于 2025-3-31 20:50:29
Applications and Usability of Interactive TVch to measure localized cAMP signals. However, given the low signal-to-noise ratio of most FRET probes and the dynamic nature of the intracellular environment, there have been marked limitations in the ability to use FRET probes to study localized signaling events within the same cell. Here, we outl
altruism
发表于 2025-4-1 00:02:17
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