敏捷
发表于 2025-3-23 12:24:17
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Expertise
发表于 2025-3-23 14:03:46
CUT&Tag for Efficient Epigenomic Profiling of Frozen Tissues,col details the steps to generate CUT&Tag libraries from fresh or frozen tissues. This CUT&Tag workflow has nine main steps: isolation of nuclei from tissues, binding of nuclei to Concanavalin A–coated beads, binding of the primary antibody, binding of the secondary antibody, binding pA-Tn5 adapter
要塞
发表于 2025-3-23 20:39:08
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Fierce
发表于 2025-3-23 22:21:19
Single-Cell Histone Modification Profiling with Cell Enrichment Using sortChIC,tiation, the distribution of histone modifications is remodeled, resulting in cell type–specific patterns. In the past, their study was limited to abundant cell types that could be purified in necessary numbers. However, studying these cell type–specific dynamic changes in heterogeneous in vivo sett
DUST
发表于 2025-3-24 04:26:45
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烧烤
发表于 2025-3-24 08:43:04
ChEC-Seq: A Comprehensive Guide for Scalable and Cost-Efficient Genome-Wide Profiling in ,nding locations in vivo, does not require antibodies or fixation, and provides genome-wide coverage at near nucleotide resolution..The core of this method is an MNase fusion of the target protein, which allows it, when triggered by calcium exposure, to cut DNA at its binding sites and to generate sm
一窝小鸟
发表于 2025-3-24 14:30:37
Chromatin Immunoprecipitation in Adipose Tissue and Adipocytes: How to Proceed and Optimize the ProIP from adipocytes or frozen adipose tissue collection, isolation of nuclei, cross-linking of protein-DNA complexes, chromatin shearing, immunoprecipitation, and DNA purification. We also discuss critical steps for optimizing the experiment to perform a successful ChIP in lipid-rich cells/tissues.
Mirage
发表于 2025-3-24 17:07:14
Bioinformatics Core Workflow for ChIP-Seq Data Analysis,t covers data processing and initial analytical steps of ChIP-seq data. We provide a step-by-step protocol of the commands as well as a fully assembled Snakemake workflow. Along the protocol, we discuss key tool parameters, quality control, output reports, and preliminary results.
良心
发表于 2025-3-24 22:16:25
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Surgeon
发表于 2025-3-25 01:39:53
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