Preamble
发表于 2025-3-25 04:12:42
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背叛者
发表于 2025-3-25 09:45:27
Coronavirus Transcription: A Perspective,n to explain how subgenome-length minus strands, which are used as templates for the synthesis of subgenomic mRNAs, might arise by a process involving discontinuous RNA synthesis. The old model explaining subgenomic mRNA synthesis, which was called leader-primed transcription, was based on erroneous
nutrients
发表于 2025-3-25 11:48:12
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FORGO
发表于 2025-3-25 18:02:29
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启发
发表于 2025-3-25 22:56:39
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自制
发表于 2025-3-26 00:08:52
Coronavirus Reverse Genetics and Development of Vectors for Gene Expression,NAs. With the transmissible gastroenteritis virus (TGEV) genome the efficient (>40 .g per 10. cells) and stable (>20 passages) expression of the foreign genes has been shown. Knowledge of the transcription mechanism in coronaviruses has been significantly increased, making possible the fine regulati
安抚
发表于 2025-3-26 07:11:41
Reverse Genetics of Coronaviruses Using Vaccinia Virus Vectors,pproach to coronavirus reverse genetics and was first described for the generation of recombinant human coronavirus 229E representing a group I coronavirus. Subsequently, the same approach has been used to generate recombinant avian infectious bronchitis coronavirus and, recently, recombinant mouse
尖叫
发表于 2025-3-26 10:18:47
Development of Mouse Hepatitis Virus and SARS-CoV Infectious cDNA Constructs,allows for the assembly of very large RNA and DNA genomes from a panel of contiguous cDNA subclones. Recombinant viruses generated from these methods contained the appropriate marker mutations and replicated as efficiently as wild-type virus. The MHV cloning strategy can also be used to generate rec
镶嵌细工
发表于 2025-3-26 15:55:37
Coronavirus Genome Structure and Replication,nonstructural proteins involved in proteolytic processing, genome replication, and subgenomic mRNA synthesis (transcription) (an estimated 14–16 end products for coronaviruses) are encoded within the 5′-proximal two-thirds of the genome on gene 1 and the (mostly) structural proteins are encoded with
Alpha-Cells
发表于 2025-3-26 16:54:10
Coronavirus Transcription: A Perspective,rmulate a third model proposing that the discontinuous event in coronavirus RNA synthesis occurs during minus strand synthesis. With our model the genome is copied both continuously to produce minus-strand templates for genome RNA synthesis and discontinuously to produce minus-strand templates for s