delusion
发表于 2025-3-30 10:25:06
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蚊帐
发表于 2025-3-30 14:00:00
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机警
发表于 2025-3-30 18:15:39
A Step-by-Step Guide to Instant Structured Illumination Microscopy (iSIM),ng twice the diffraction limit. Here we briefly describe the theory of iSIM and outline a typical hardware setup. We also provide step-by-step guides for generating a cellular-based fluorescent standard, obtaining a multicolor image with iSIM, and the post-processing steps of de-striping and deconvo
乐意
发表于 2025-3-30 20:51:26
1064-3745 ation advice from the experts.This volume provides a wide range of imaging protocols that can be tailored to specific organisms or cell-types. Chapters guide readers through fixed-cell, live-cell, phenotype screening, super-resolution, intravital imaging techniques, and fluorescence life-time imagi
Catheter
发表于 2025-3-31 03:54:37
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EVADE
发表于 2025-3-31 08:03:15
opy. This basic protocol steps the user through a typical three-color imaging experiment using a Zeiss LSM 880 confocal microscope for the example. The extensive Notes section attempts to generalize the method so that concepts and considerations can be applied to other laser scanning confocal systems.
伙伴
发表于 2025-3-31 12:50:25
reorganization of the actin cytoskeleton leading both the directional cell movement and the engulfment of bacteria. Many live cell imaging methods have been developed and applied to monitor these signaling events. In this chapter, we will introduce how to measure GPCR-mediated signaling events for cell migration and phagocytosis in ..
整洁漂亮
发表于 2025-3-31 15:08:42
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manifestation
发表于 2025-3-31 18:03:55
Choosing Fluorescent Probes and Labeling Systems,he protein of interest. The advantages and disadvantages of different fluorescent probe types and their respective labeling methods are discussed to provide an overview on selecting appropriate fluorophores and labeling systems for fluorescence-based assays. Protocols are outlined when appropriate.
强制令
发表于 2025-4-1 00:58:16
General Considerations for Acquiring a Three-Color Image by Laser Scanning Confocal Microscopy,opy. This basic protocol steps the user through a typical three-color imaging experiment using a Zeiss LSM 880 confocal microscope for the example. The extensive Notes section attempts to generalize the method so that concepts and considerations can be applied to other laser scanning confocal systems.