nocturia 发表于 2025-3-28 15:34:36
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Analysis of Rhythmic Gene Expression in Adult , Using the Firefly Luciferase Reporter Genected during a specific time of the circadian day need to be sacrificed, followed by the extraction of clock gene products. This procedure makes it impossible to analyze molecular rhythms in an individual over time. To measure clock gene expression within the living animal, firefly luciferase can beairborne 发表于 2025-3-29 00:17:58
Monitoring Circadian Rhythms in , Using Luciferase Reporter Genesnot display any obvious circadian rhythms; however, rhythms in gene expression can be readily detected. For this reason the promoters of rhythmically expressed genes have been fused to the firefly . gene. Using this reporter gene we have developed a number of automated techniques for monitoring luci微生物 发表于 2025-3-29 04:03:28
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Mutagenesis With , . pacemaker genes were discovered through the isolation of gene variants affecting the free-running period of the circadian pacemaker. There are different ways to mutagenize flies. An alkylating agent can be used to randomly alter the fly genome, or transposable elements can be mobilized to disrupt不满分子 发表于 2025-3-29 19:31:07
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Microarrayshis technology, it is important to perform quality control experiments before engaging in genome-wide expression analysis. In this chapter, we provide an overview of quantitative polymerase chain reaction experiments using the ABI PRISM® 7900HT system for quality control of samples, and micorarray eFluctuate 发表于 2025-3-30 04:56:39
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