无法治愈 发表于 2025-3-28 18:18:21
,Site-Specific Chromosomal Integration Mediated by ϕC31 Integrase,ise, unidirectional recombination between two attachment or . sites called . and .. We have shown that an . site preintegrated into a mammalian chromosome can serve as a target for integration of an introduced plasmid carrying an . site. Recombination leads to precise integration of the plasmid intodegradation 发表于 2025-3-28 21:04:36
http://reply.papertrans.cn/23/2264/226334/226334_42.png文字 发表于 2025-3-29 02:39:11
http://reply.papertrans.cn/23/2264/226334/226334_43.png圆锥 发表于 2025-3-29 03:37:33
Mycobacterial Recombineering, continues to be hindered by problems of relatively poor DNA uptake, slow growth rate, and high levels of illegitimate recombination. In . an effective approach to stimulating recombination frequencies has been developed called “recombineering,” in which phage-encoded recombination functions are traIntend 发表于 2025-3-29 07:38:13
Chromosomal Engineering of , Using Group II Introns,studying the role of toxins in disease pathogenesis. However, it has been very difficult to introduce mutations into .. We recently developed a clostridia-modified targetron that can specifically and efficiently inactivate . genes. The usefulness of this system has now been demonstrated by specificaNausea 发表于 2025-3-29 12:43:55
http://reply.papertrans.cn/23/2264/226334/226334_46.pngIOTA 发表于 2025-3-29 19:27:25
http://reply.papertrans.cn/23/2264/226334/226334_47.pngdaredevil 发表于 2025-3-29 22:49:32
Taiwanese Collaborative Product Commercehnologies, gene knockout studies using human somatic cells will be of greater importance for analyzing the functions of human genes in greater detail. Although the frequency of gene targeting is typically very low in human cultured cells, we have recently shown that a human precursor B cell line, NaPLUMP 发表于 2025-3-30 03:39:33
The Scandinavian Approach to mBankingcell lines can be engineered with meganucleases, sequence-specific endonucleases that recognize large DNA target sites. These proteins are powerful tools for genome engineering because they can increase homologous gene targeting by several orders of magnitude in the vicinity of their cleavage site.周年纪念日 发表于 2025-3-30 06:18:36
http://reply.papertrans.cn/23/2264/226334/226334_50.png