Clinch
发表于 2025-3-25 04:22:10
Key Concepts in Chinese Thought and Cultureoaches that efficiently reduce the expression of these transcripts. Here, I describe a method that allows a specific and efficient targeting of the highly abundant lncRNA . in human (lung) cancer cells. The method relies on the site-specific integration of RNA-destabilizing elements mediated by Zinc Finger Nucleases (ZFNs).
antidote
发表于 2025-3-25 08:52:27
Redefining Chinese Literature and Art enrich for genome-edited cells such that they can be more easily identified. This chapter discusses several different approaches including the use of cold-shock, exonucleases, surrogate markers, specialized donor vectors, and oligonucleotides to enhance the frequency of genome editing or to facilitate the identification of genome-edited cells.
NUL
发表于 2025-3-25 12:44:35
https://doi.org/10.1007/978-1-4899-0961-9aced short palindromic repeats (CRISPR)-Cas9. These endonucleases are useful for simple and rapid production of gene knockout/knockin animals without using embryonic stem (ES) cells. This chapter introduces the latest protocols for producing genetically modified rodents using ZFN, TALEN, and CRISPR/Cas9.
notification
发表于 2025-3-25 16:42:26
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相一致
发表于 2025-3-25 21:53:28
Strategies to Increase Genome Editing Frequencies and to Facilitate the Identification of Edited Ce enrich for genome-edited cells such that they can be more easily identified. This chapter discusses several different approaches including the use of cold-shock, exonucleases, surrogate markers, specialized donor vectors, and oligonucleotides to enhance the frequency of genome editing or to facilitate the identification of genome-edited cells.
细菌等
发表于 2025-3-26 02:37:36
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过去分词
发表于 2025-3-26 08:06:51
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议程
发表于 2025-3-26 11:37:06
Introduction: Political Cultures,g tools that are amenable to multiplexing and compatible with multiple cellular delivery methods. In this chapter, we describe the process of assembling a megaTAL from a meganuclease, as well as a method for characterization of nuclease cleavage activity in vivo using a fluorescence reporter assay.
预感
发表于 2025-3-26 15:21:30
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Comprise
发表于 2025-3-26 19:13:04
Adele Eskeles Gottfried,Allen W. Gottfriedsing liquid nitrogen. This chapter introduces our latest protocols for freeze-drying of mouse and rat spermatozoa, and the anticipated results of the fertilizing ability of these gametes following long-term preservation or transportation.