Constituent
发表于 2025-3-26 21:22:44
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钝剑
发表于 2025-3-27 04:28:35
Lecture Notes in Physics Monographss a technique in which a target protein of interest bound by an antibody is used to pull down the protein complex out of cell lysates, which can be identified by mass spectrometry. Here, we describe the protocol to immunoprecipitate and identify the components of the protein complexes of ElmoE in . cells.
得体
发表于 2025-3-27 05:49:03
Review of Relativistic Field Theory,rom transgenic flies expressing fluorescent proteins in the border cells, and using confocal microscopy to take a multi-positional time-lapse movie. We include an image analysis method for tracking border cell cluster dynamics as well as tracking individual cell movements.
CYT
发表于 2025-3-27 11:41:46
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调整
发表于 2025-3-27 14:24:18
In Situ Assays of Chemotropism During Yeast Mating, two haploid cells of opposite mating type grow towards one another. Mating pheromone gradients are essential for efficient mating in yeast and different yeast mutants are defective in chemotropism. Two methods of assessing the ability of yeast strains to respond to pheromone gradients are presented here.
手铐
发表于 2025-3-27 20:14:40
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遣返回国
发表于 2025-3-28 00:13:48
Studying Chemoattractant Signal Transduction Dynamics in , by BRET, transduction is the social amoeba .. However, the methods available to quantitatively measure chemotactic signaling are limited. Here, we describe a protocol to quantitatively study chemoattractant signal transduction in . by monitoring protein–protein interactions and conformational changes using Bioluminescence Resonance Energy Transfer (BRET).
闪光你我
发表于 2025-3-28 06:04:59
Identification of Associated Proteins by Immunoprecipitation and Mass Spectrometry Analysis,s a technique in which a target protein of interest bound by an antibody is used to pull down the protein complex out of cell lysates, which can be identified by mass spectrometry. Here, we describe the protocol to immunoprecipitate and identify the components of the protein complexes of ElmoE in . cells.
参考书目
发表于 2025-3-28 06:27:09
Live Imaging of Border Cell Migration in ,,rom transgenic flies expressing fluorescent proteins in the border cells, and using confocal microscopy to take a multi-positional time-lapse movie. We include an image analysis method for tracking border cell cluster dynamics as well as tracking individual cell movements.
时间等
发表于 2025-3-28 13:41:22
Flow Cytometry-Based Quantification of HIV-Induced T Cell Chemotactic Response,which are necessary for viral nuclear migration and the latent infection of blood resting CD4 T cells. In this chapter, we describe the methods for quantification of HIV-induced actin polymerization and cofilin phosphorylation in human T cells using flow cytometry.