ARSON 发表于 2025-3-21 19:02:12
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Detecting Posttranslational Modifications of Hsp90 Isoforms,modifications (such as phosphorylation, SUMOylation, and ubiquitination) are important for Hsp90 stability and regulation of its ATPase activity. Both mammalian and yeast cells can be used to express and purify Hsp90 and TRAP1 and also detect post-translational modifications by immunoblotting.委派 发表于 2025-3-22 08:43:30
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Book 2023Latest editionps on troubleshooting and avoiding known pitfalls..Cutting-edge and thorough, .Chaperones: Methods and Protocols, Second Edition. is a valuable resource for all researchers who want to learn more about this interesting and developing field. .delusion 发表于 2025-3-23 05:00:54
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Practical Ajax Projects with Java Technologym isolated nuclei and sheared by sonication. Hsp90-bound loci are immunoprecipitated and the corresponding DNA fragments are purified and sequenced. The described approach revealed that Hsp90 binds close to the transcriptional start site of around one-third of all . coding genes and characterized the role of the chaperone at chromatin.