赦免
发表于 2025-3-28 18:14:13
Mutation and Mutation Screening,nts in the environment); and mutation induction requires an understanding and assessment of the appropriate mutagen dose required. The techniques of mutation induction, dose optimization, and TILLING are explained.
LIKEN
发表于 2025-3-28 21:55:53
The Quantitative Real-Time Polymerase Chain Reaction for the Analysis of Plant Gene Expression,on to fundamental concepts relevant for the analysis of gene expression in plants using this technique and a protocol for quantification of the relative expression of a sucrose phosphate synthase gene along the maturation gradient of a sugarcane leaf.
火海
发表于 2025-3-29 00:26:46
Genome Walking,n to the characterized species. The increasing use of next-generation sequencing technologies will see the principles of genome walking adapted to . methods. However, for smaller projects, PCR-based genome walking will remain an efficient method of characterizing unknown flanking sequence.
ACRID
发表于 2025-3-29 05:49:18
Functional Analysis by Protein Biochemistry,for expression of plant genes, followed by purification and in vitro characterization of the resultant enzyme. The second section of this chapter details the methods involved in transient gene expression in . leaf protoplasts for in vivo functional characterization of protein localization.
CHAR
发表于 2025-3-29 09:00:44
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Mechanics
发表于 2025-3-29 11:42:18
Protein Blotting Protocol for Beginners, blotting involves five steps, namely, sample collection, preparation, separation, immobilization, and detection. In this chapter, protocols for the entire process from sample collection to detection are described.
矛盾心理
发表于 2025-3-29 17:53:01
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dictator
发表于 2025-3-29 23:03:58
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清澈
发表于 2025-3-30 02:17:14
https://doi.org/10.1007/978-3-658-26256-3traction process and downstream reactions. Thus the removal of starch by using appropriate methods is necessary for obtaining pure RNA to be processed for functional genomics analysis. We describe a method to isolate large amount of good-quality RNA from developing and mature wheat grain which can also be adapted to other cereal grains.
纯朴
发表于 2025-3-30 04:15:01
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