libertine 发表于 2025-3-26 23:05:41

ICU Staffing, Models, and Outcomestein produced as inclusion bodies in . can be expressed as a soluble and active protein in an .-based cell-free expression system, such as the RTS . However, if aggregation also occurs in cell-free systems, the conditions can easily be adjusted to overcome the problem. For example, the addition o

细菌等 发表于 2025-3-27 02:20:06

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关节炎 发表于 2025-3-27 07:21:36

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Obstreperous 发表于 2025-3-27 12:53:25

Improved Batch Translation System Based on , Extract (SFCF) reactor was achieved. The results document that it is possible to avoid the limitations of the ultrafiltration membranes during product removal from a CFCF reactor. The data presented show that the affinity system is also well suited for the development of a novel protein bioreactor.

上腭 发表于 2025-3-27 15:17:33

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滑稽 发表于 2025-3-27 20:03:32

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通便 发表于 2025-3-27 22:43:49

Co-Translational Protein Folding in Prokaryotic and Eukaryotic Cell-Free Translation Systemst peptide starts its folding as soon as it is synthesized and emerges from the ribosome prior to the formation of the entire polypeptide chain (see refs below). The existence of stepwise protein folding starting from the N-terminal section of a growing polypeptide and progressively proceeding toward

indoctrinate 发表于 2025-3-28 04:34:00

Expression of an Aggregation-Prone Protein in the RTS 500 Systemch serves as periplasmic receptor for the high-affinity transport of maltodextrins and for a defective MalE folding mutant, MalE31, that forms inclusion bodies when expressed in bacteria (Betton and Hofnung 1996).

澄清 发表于 2025-3-28 09:10:11

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诱惑 发表于 2025-3-28 10:42:49

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查看完整版本: Titlebook: Cell-Free Translation Systems; Alexander S. Spirin Book 2002 Springer-Verlag Berlin Heidelberg 2002 Expression.Polymerasekettenreaktion.Pr