指责 发表于 2025-3-21 17:51:18
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Analysis of the Budding Yeast , Cell Cycle by Morphological Criteria and Flow Cytometryplex methodology as fluorescence-activated cell sorting (FACS) can be readily adapted with additional calibration for the small cell size and the relatively low deoxyribonucleic acid (DNA) content of diploid or haploid yeast. In the following, we have compiled protocols for some of the most common m骂人有污点 发表于 2025-3-22 15:30:23
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https://doi.org/10.1007/978-94-015-8426-5very good. The first limitation can be overcome by plating multiple T150 flasks with cells, using roller bottles, or blocking cells in mitosis by inhibitors such as Colcemid or nocodazole (.). Mitotic cells that are collected can be held on ice for an hour or so while multiple collections are done t缺乏 发表于 2025-3-23 08:28:41
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