pulse-pressure 发表于 2025-3-23 10:19:08
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Preparation of , Seedling Proteomes for Identifying Metacaspase Substrates by N-terminal COFRADIC by comparing the N-terminal proteomes (or N-terminomes) of wild-type plants to transgenic plants not expressing a given metacaspase. In this chapter we describe a protocol for the preparation of plant tissue proteomes, including differential isotopic labelling allowing for a comparison of in vivo Nantiquated 发表于 2025-3-24 03:00:16
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Peter V. Bozhkov,Guy SalvesenIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the expert.Includes supplementary material缓和 发表于 2025-3-24 14:54:50
Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/222387.jpgGUILT 发表于 2025-3-24 22:52:53
Positional Scanning Substrate Combinatorial Library (PS-SCL) Approach to Define Caspase Substrate Spscribe the protocol for analyzing S4-S2 pockets preferences of caspases using PS-SCL. Additionally, we describe procedures for the identification of optimal substrates sequence after PS-SCL, solid phase synthesis, and purification of selected fluorogenic substrates, as well as their kinetic analysis.Ptsd429 发表于 2025-3-25 03:10:48
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