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Endothelial and fibroblast viability assays for tissue allograftsthe primary consideration. The viability of any tissue after cryopreservation is dependent in part upon handling during procurement and prefreezing storage. Any exposure to non-physiological conditions, such as ischemia, hypoxia, or anoxia causes direct toxicity to most cell types or sensitizes the
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A. Charles Yankah (Consultant),Roland Hetzer (Prof
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fts, as well as their clinical use. No doubt, at present, cardiac valve allografts of one sort or another are the devices of choice for conduits and have an important place in the surgery of aortic valve replacement. Even so, in the mind of this writer at least, the future usefulness of allografts for the rep978-3-642-72422-0978-3-642-72420-6
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GLUT
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Contribution for discussion: Heart valve procurement in Berlinal reasons. In December 1986, the Berlin Heart Valve Procurement Program was initiated by the German Heart Center (2). Since then, two main sources for heart valve procurement have been established: (1) heart transplant recipients and (2) multi-organ donors.