火光在摇曳 发表于 2025-3-28 16:04:46
http://reply.papertrans.cn/23/2216/221511/221511_41.png六个才偏离 发表于 2025-3-28 21:49:04
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http://reply.papertrans.cn/23/2216/221511/221511_43.pngABHOR 发表于 2025-3-29 04:49:00
Space Geometry from Ancient Greece to Todayg phenotype-associated chromatin 3D signatures. Here, I describe a detailed protocol of targeted DNase Hi-C library preparation, which covers experimental steps starting from cell cross-linking to library amplification.土产 发表于 2025-3-29 07:40:49
http://reply.papertrans.cn/23/2216/221511/221511_45.png曲解 发表于 2025-3-29 14:48:02
,Pilze, Lebenspartner der Waldbäume,n-mediated amplification (LMA) followed by deep sequencing to quantitatively detect specified genomic regions in ChIP samples. Here, we describe the generation of 2C-ChIP libraries and computational processing of the resulting sequencing data.假装是你 发表于 2025-3-29 17:39:59
https://doi.org/10.1007/978-1-4302-3991-8hogens..Experimental treatments often require the isolation of cells from their physiological environment, exposing them to possible sudden changes in their nuclear architecture. Here we propose an early cross-linking on primary cells, a fixing method that can help to minimize the risk of nuclear st小样他闲聊 发表于 2025-3-29 21:16:07
http://reply.papertrans.cn/23/2216/221511/221511_48.png羊栏 发表于 2025-3-30 03:16:00
Best Practices im No-Line-Handel,muno-DNA FISH detection of genes and Human immunodeficiency virus 1 (HIV-1) provirus in primary CD4. T cells from healthy donors, or cells infected in vitro with the virus. Our multicolor 3D-FISH technique allows, by using up to three fluorophores, visualization of spatial positioning of loci insideVeneer 发表于 2025-3-30 06:40:37
https://doi.org/10.1007/978-1-4684-1467-7imary T cells. We believe that our protocol will contribute to the improvement of epigenetic studies on the 3D nuclear structure of T cell subsets, possibly shedding light on the still hidden epigenetic players responsible for the great plasticity and functional diversification exerted by T cells.