小虫
发表于 2025-3-30 08:56:33
Efficient Homologous Recombination-Mediated in Planta Gene Targeting by Egg-Cell-Specific Expressioependent of high transformation efficiencies. Here, we describe an updated protocol of ipGT for the model plant Arabidopsis, taking into account our recent improvements based on egg-cell-specific expression of . Cas9.
教唆
发表于 2025-3-30 14:04:39
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pulmonary
发表于 2025-3-30 16:53:09
Homology-Directed Transgene-Free Gene Editing in ,possibility to purchase all necessary reagents commercially with no preparation time (besides design) facilitates rapid and routine genetic engineering in this organism. Here we describe the use of this technique to knockout locus ., which leads to rapamycin resistance and lends itself to an easy assay when adopting this gene-editing protocol.
Mingle
发表于 2025-3-31 00:05:05
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传授知识
发表于 2025-3-31 03:52:39
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MORPH
发表于 2025-3-31 07:13:12
Die Entfaltung der Wirbeltiere, system over other transformation methods. In this chapter, we describe a detailed protocol for generating . mutants via AMT to deliver single-guide RNA and Cas9, the components required for gene editing. This protocol can be used directly for CRISPR-Cas9-mediated gene editing of . and modified for other culturable oomycete species.