Thyroiditis
发表于 2025-3-25 03:30:38
https://doi.org/10.1007/978-3-322-95767-2es on the ability of ascorbate to reduce the covalent bond between the NO radical and the protein, allowing the exchange of the NO radical with a thiol reactive biotin-HPDP. The biotinylated proteins are then easily purified by using NeutrAvidin resin, separated by SDS-PAGE resolution and analyzed by Western blotting.
CROW
发表于 2025-3-25 11:11:38
Exploration of Fas S-Nitrosylation by the Biotin Switch Assay,es on the ability of ascorbate to reduce the covalent bond between the NO radical and the protein, allowing the exchange of the NO radical with a thiol reactive biotin-HPDP. The biotinylated proteins are then easily purified by using NeutrAvidin resin, separated by SDS-PAGE resolution and analyzed by Western blotting.
intercede
发表于 2025-3-25 14:35:06
Thomas Koszinowski,Hanspeter Mattestic separation by western blotting or mass spectrometry. Herein, we describe in detail the method used in our laboratory to immunoprecipitate and analyze by immunoblot complexes containing caspase-8, using a commercial antibody directed against caspase-8.
Prophylaxis
发表于 2025-3-25 16:23:58
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concise
发表于 2025-3-25 23:50:09
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BURSA
发表于 2025-3-26 03:34:43
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补充
发表于 2025-3-26 07:19:51
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路标
发表于 2025-3-26 09:53:28
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实施生效
发表于 2025-3-26 16:07:16
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Ingenuity
发表于 2025-3-26 19:40:32
Thomas Koszinowski,Hanspeter Mattes components of the IRF3 pathway are found at the Golgi apparatus. Here we describe simple methods to observe and assess these ubiquitylated components by immunoblotting using differential centrifugation and in vitro assays.