ULCER 发表于 2025-3-23 12:37:48
978-1-4939-8197-7Springer Science+Business Media New York 2017藐视 发表于 2025-3-23 15:16:10
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Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/220234.jpg果仁 发表于 2025-3-23 23:11:51
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https://doi.org/10.1007/978-3-663-07312-3sented. In this method, embryos are hybridized with Dig-labeled riboprobes, and the riboprobes are detected by use of the alkaline-phosphatase reaction in the presence of a 4-nitro-blue tetrazolium chloride and 5-bromo-4-chloro-3-indolyl-phosphate (NBT + BCIP) mixture. Obvious detection of positive爱管闲事 发表于 2025-3-24 09:49:22
https://doi.org/10.1007/3-540-28495-8erse transcriptase PCR (qPCR) quantifies mRNA levels of these key genes. Immunostaining localizes CCN proteins within skin tissue, and provides semiquantitative information regarding relative levels of the proteins. Reliable determinations of the expression levels of CCN genes and proteins are essentroponins 发表于 2025-3-24 14:05:14
Zusammenfassung und Schlussfolgerungen,mounts of specific mRNA transcripts, done by measuring amplified levels of specific cDNAs. In this chapter, we provide our real-time RT-PCR experimental procedure using SYBR Green I for the quantitative analysis of CCN family gene expression. Especially, we describe the extraction and purification sGrandstand 发表于 2025-3-24 18:41:09
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Zusammenfassung und Schlussfolgerungen,s. These techniques give good results on cell culture but for organs that are made of numerous cells with several compartments, various states of differentiation as in epidermis, immunohistochemistry is always relevant. Double (triple) staining by immunofluorescence allows positive cells identificat