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Isolation of Plasma Membrane Sheets and Plasma Membrane Domains from Rat Liver,: basolateral and apical. In hepatocytes, the basolateral domain includes the sinusoidal front, which faces the space of Disse and is involved in exchange of metabolites with the circulation, and the lateral surface, which is contiguous to neighboring hepatocytes. The apical or bile canalicular doma多骨 发表于 2025-3-22 10:35:23
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The Isolation of Clathrin-Coated Vesicles and Purification of Their Protein Components,ort of newly synthesized lysosomal enzymes from the trans-Golgi network, and at the plasma membrane they are responsible for receptor-mediated endocytosis of selected ligands (.). Coated vesicles are the best characterized of all transport organelles, owing in large measure to the ease with which thTruculent 发表于 2025-3-22 17:49:13
Isolation of Membranes from Tissue Culture Cells,ticularly difficult with suspension culture cells, as opposed to those that grow as an adherent monolayer. The choice of subcellular fractionation procedure depends on the results of the homogenization. For a review of the problems associated with the isolation of plasma membranes from tissue culturuncertain 发表于 2025-3-22 22:37:35
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Isolation and Purification of Functionally Intact Chloroplasts from Leaf Tissue and Leaf Tissue Protron flow and phosphorylation, metabolite transport, and protein targeting. Whatever the subsequent use of the chloroplasts, it is preferable to attempt to isolate . chloroplasts, as the organelle envelope will protect the stroma and thylakoids from the deleterious effects of degradative enzymes and巨硕 发表于 2025-3-23 08:27:47
Isolation and Purification of Functionally Intact Mitochondria from Plant Cells,onsiderable expertise. Although it is relatively easy to prepare a crude mitochondrial fraction by differential centrifugation, marked changes in functional capabilities and morphological appearance are often observed in such fractions, suggesting that some degree of structural damage has occurred d