PANEL 发表于 2025-3-30 10:53:28
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,Schlußbetrachtungen und Ausblick,s in living cells under physiological conditions. Here we describe protocols for these assays including relevant positive and negative controls, and we also show how they can be combined with protein complementation assays such as bimolecular fluorescence complementation (BiFC) to study three- and f药物 发表于 2025-3-31 10:30:44
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Luminescent Probes and Visualization of Bioluminescence,e discovery of luciferin and luciferases, molecular biology techniques allowed the cloning of several luciferases and photoproteins. Among most used BL reporters, we find firefly and click-beetle luciferases, bacterial luciferase, ., ., and . luciferases, and calcium-activated photoproteins. Accordi垄断 发表于 2025-3-31 18:47:14
Validation of Bioluminescent Imaging Techniques,esearch, cell transplantation, and monitoring of infectious disease in which bioluminescence intensity is correlated with other metrics. However, bioluminescence measurements can be influenced by a number of factors, among them source location, tissue optical properties, and substrate availability a带子 发表于 2025-3-31 22:59:08
Assessment of Extracellular ATP Concentrations,l surface purinergic receptors (purinoceptors). Biological responses regulated by purinergic receptors include neurotransmission, smooth muscle relaxation and contraction, epithelial cell ion transport, inflammation, platelet activation, immune responses, cardiac function, endocrine and exocrine sec