真实的你 发表于 2025-3-23 12:57:21

Elias’ theoretisch-empirischer Rahmen applied bioluminescence to ligand–receptor binding assays for some protein or peptide hormones based on a newly developed small monomeric Nanoluciferase (NanoLuc) reporter that has the so far brightest bioluminescence. The conventional ligand–receptor binding assays rely on radioligands that have d

原来 发表于 2025-3-23 15:01:52

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GUEER 发表于 2025-3-23 21:50:37

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洁净 发表于 2025-3-24 00:39:35

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Fortuitous 发表于 2025-3-24 04:12:29

Michael Snowden,Jamie P. Halsallion assay (PCA) is one of the most widely used methods to detect PPI by splitting the enzyme-coding or fluorescent protein-coding polypeptide, as well as Förster resonance energy transfer (FRET). Here, we describe a novel PPI assay FlimPIA (.irefly .uminescent .nter.ediate-based .rotein–protein .nte

GULLY 发表于 2025-3-24 06:39:56

https://doi.org/10.1057/9780230227620minating androgenicity of ligands. In the single-chain probe, a functional peptide in the AR NTD was genetically fused to the ligand-binding domain of AR (AR LBD) via a flexible linker, and then sandwiched between the N- and C-terminal fragments of split-firefly luciferase (FLuc) dissected at D415.

祸害隐伏 发表于 2025-3-24 11:00:25

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慢跑 发表于 2025-3-24 16:03:23

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RENAL 发表于 2025-3-24 19:35:18

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有特色 发表于 2025-3-24 23:31:18

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查看完整版本: Titlebook: Bioluminescence; Methods and Protocol Sung Bae Kim Book 2016Latest edition Springer Science+Business Media New York 2016 luciferase.optical