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发表于 2025-3-21 17:33:23
书目名称Basic Techniques in Molecular Biology影响因子(影响力)<br> http://impactfactor.cn/if/?ISSN=BK0181172<br><br> <br><br>书目名称Basic Techniques in Molecular Biology影响因子(影响力)学科排名<br> http://impactfactor.cn/ifr/?ISSN=BK0181172<br><br> <br><br>书目名称Basic Techniques in Molecular Biology网络公开度<br> http://impactfactor.cn/at/?ISSN=BK0181172<br><br> <br><br>书目名称Basic Techniques in Molecular Biology网络公开度学科排名<br> http://impactfactor.cn/atr/?ISSN=BK0181172<br><br> <br><br>书目名称Basic Techniques in Molecular Biology被引频次<br> http://impactfactor.cn/tc/?ISSN=BK0181172<br><br> <br><br>书目名称Basic Techniques in Molecular Biology被引频次学科排名<br> http://impactfactor.cn/tcr/?ISSN=BK0181172<br><br> <br><br>书目名称Basic Techniques in Molecular Biology年度引用<br> http://impactfactor.cn/ii/?ISSN=BK0181172<br><br> <br><br>书目名称Basic Techniques in Molecular Biology年度引用学科排名<br> http://impactfactor.cn/iir/?ISSN=BK0181172<br><br> <br><br>书目名称Basic Techniques in Molecular Biology读者反馈<br> http://impactfactor.cn/5y/?ISSN=BK0181172<br><br> <br><br>书目名称Basic Techniques in Molecular Biology读者反馈学科排名<br> http://impactfactor.cn/5yr/?ISSN=BK0181172<br><br> <br><br>
PIZZA
发表于 2025-3-21 20:26:59
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渐变
发表于 2025-3-22 02:08:16
Preparation of Genomic DNA from Plant Cells, from animal or bacterial cells. Plant cells have a very tough cell wall, the breakage of which requires the application of vigorous physical force that can shear large DNA molecules. Also, plant cells accumulate large amounts of sencondarymetabolities in their vacuoles that either co-purify with nu
他日关税重重
发表于 2025-3-22 07:20:43
Isolation of Plasmid DNA, In most instances, plasmid DNA, isolated by these procedures, is of sufficient quality and quantity for cloning and PCR analysis. However, the plasmid DNA obtained by many of these methods is a poor template for double-stranded dideoxy DNA sequencing, largely due to inconsistency of template purity
Host142
发表于 2025-3-22 09:32:53
Isolation and Purification of RNA,, intact RNA is one of the central techniques in molecular biology and represents an important step in Northern analysis, nuclease protection assays, RNA mapping, RT-PCR, cDNA library construction and in vitro translation experiments.
使服水土
发表于 2025-3-22 13:56:21
Isolation of PolyA+ RNA,emaining being ribosomal RNA and various species ofsmall stable RNAs. For some applications, such as construction of cDNA libraries or analysis of low-abundance mRNAs, isolation of mRNA instead oftotal cellular RNA is essential.
托人看管
发表于 2025-3-22 19:13:08
Agarose Gel Electrophoresis of DNA,influencing the optimal separation of DNA bands in agarose gels. Protocols for running standard agarose gels and high resolution agarose gels are given, as well as a method for recovery of DNA fragments from the gel.
Pandemic
发表于 2025-3-22 21:38:02
Preparation of Probes for Hybridization,t one or both ends of nucleic acid molecules, giving specific, low density labeled probes. High density labeling is usually achieved by incorporating the reporters uniformly throughout entire length of nucleic acid molecules. For hybridization work internally labeled probes are preferred since they
组装
发表于 2025-3-23 01:50:39
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COMMA
发表于 2025-3-23 08:33:50
DNA Transfer and Hybridization, technique permits hybridization of various probes to immobilized target DNA under controlled conditions, coupled with fast detection of the hybrid. Because of the sensitivity, speed, and convenience of this “solid state” hybridization procedure, it has been widely applied in applied and basic resea