Nefarious
发表于 2025-3-26 23:14:26
Northern Blot Analysislar in vivo system being examined. This chapter details the method for analyzing RNA by Northern blotting, which basically involves the isolation of RNA, its size fractionation by electrophoresis, transfer to membrane, and detection by nucleic acid hybridization and autoradiography.
极少
发表于 2025-3-27 02:43:27
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贝雷帽
发表于 2025-3-27 06:34:55
Techniques in Molecular Hematology, the enzymes are highly processive, allowing the efficient synthesis of very long transcripts from DNA templates. In this chapter, the preparation of the DNA templates and the transcription from the template of .P-labeled synthetic RNA molecules, commonly called riboprobes, will be discussed.
Dawdle
发表于 2025-3-27 12:35:10
H. J. Deeg,D. T. Bowen,C. Niemeyer-stranded DNA probes were used for the assay (.). However, these have the disadvantage of being lengthy to prepare. The ease with which labeled RNA molelcules (riboprobes; ..) can now be made makes an assay based on RNA RNA hybridization much more favorable (.,.).
水獭
发表于 2025-3-27 17:39:26
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Conduit
发表于 2025-3-27 18:33:19
The Preparation of Riboprobes the enzymes are highly processive, allowing the efficient synthesis of very long transcripts from DNA templates. In this chapter, the preparation of the DNA templates and the transcription from the template of .P-labeled synthetic RNA molecules, commonly called riboprobes, will be discussed.
有效
发表于 2025-3-28 01:48:54
The RNase Protection Assay-stranded DNA probes were used for the assay (.). However, these have the disadvantage of being lengthy to prepare. The ease with which labeled RNA molelcules (riboprobes; ..) can now be made makes an assay based on RNA RNA hybridization much more favorable (.,.).
人充满活力
发表于 2025-3-28 02:30:19
https://doi.org/10.1385/1592590748er sensitivity applications, and the combination of fluorescein-labeling and enhanced chemiluminescent detection allows as little as 20 × 10. mol of homologous target to be detected within a 1-h exposure.
facetious
发表于 2025-3-28 09:40:27
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组装
发表于 2025-3-28 14:01:28
Hematologic Cytology of Storage Diseasesatured by sequential soaking of the gel in solutions containing HCl and NaOH, respectively. The denatured DNA fragments are then transferred to a solid matrix or filter (usually a nylon membrane) for subsequent hybridization to a specific labeled probe (.).