同义联想法
发表于 2025-3-26 23:38:31
Highly Sensitive Radioactivity-Based DNA 3′-Phosphatase Activity Assay for Polynucleotide Kinase 3′-air process. Here we describe two novel assay systems to detect phosphate release by PNKP’s 3′-phosphatase activity and PNKP-mediated in vitro single-strand break repair with minimal repair components (PNKP, DNA polymerase, and DNA ligase) using either purified proteins or cell-free nuclear extracts
孤独无助
发表于 2025-3-27 03:45:10
http://reply.papertrans.cn/19/1810/180910/180910_32.png
天文台
发表于 2025-3-27 07:52:15
In Vitro Reconstitutive Base Excision Repair (BER) Assayed in the base excision repair pathway. In the present chapter, we describe the detailed methodology to reconstitute base excision repair assay systems. These reconstitutive BER assay systems use artificially synthesized and modified DNA. These reconstitutive assay system will be a true representati
秘传
发表于 2025-3-27 13:28:17
http://reply.papertrans.cn/19/1810/180910/180910_34.png
enfeeble
发表于 2025-3-27 14:24:02
Slot Blot Assay for Detection of R Loops not always useful for initial assessments of R loop accumulation. Here, we describe an improved slot blot protocol to detect and estimate R loops and show its sensitivity and specificity using the S9.6 antibody. Since specific factors protecting cells from harmful R loop accumulation are expanding,
nerve-sparing
发表于 2025-3-27 21:50:17
Characterizing the Repair of DNA Double-Strand Breaks: A Review of Surrogate Plasmid-Based Reporteror oligonucleotide-based substrates contain restriction enzyme-cleaved DSB mimics, which undoubtedly do not mimic DSB ends generated by ionizing radiation (IR), chemotherapeutics, and reactive oxygen species (ROS). DSBs can also be indirectly generated by reactive oxygen species (ROS). All such DSB
盘旋
发表于 2025-3-28 01:57:58
http://reply.papertrans.cn/19/1810/180910/180910_37.png
矿石
发表于 2025-3-28 04:10:39
1064-3745 ips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Base Excision Repair Pathway: Methods and Protocols. serves as a valuable r978-1-0716-3375-5978-1-0716-3373-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
neuron
发表于 2025-3-28 09:14:03
http://reply.papertrans.cn/19/1810/180910/180910_39.png
左右连贯
发表于 2025-3-28 13:54:45
http://reply.papertrans.cn/19/1810/180910/180910_40.png