Indolent
发表于 2025-3-26 23:55:07
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Expostulate
发表于 2025-3-27 04:58:35
Kernel and Nonlinear Correlationsysis of NGS data developed by Luca Pinello group, DeepBaseEditor for prediction of target efficiency developed by Hyongbum Henry Kim group, and BE-Hive for prediction of target outcome developed by David Liu group.
领先
发表于 2025-3-27 07:39:16
Introduction and Perspectives of DNA Base Editorse fact that they can perform efficient and precise gene editing without generating a DNA double-strand break (DSB) or requiring a donor DNA template. Since they were first developed, significant efforts have been made to improve DNA base editors in order to overcome problems such as off-target edits
钩针织物
发表于 2025-3-27 11:14:20
Web-Based Computational Tools for Base Editorsrs with different substitution patterns, editing windows, and protospacer adjacent motif (PAM) sequences. For the design of target sequences, consideration of off-target sequences is required. In addition, for assessment of base editing outcomes in bulk populations, the analysis of high-throughput s
Pillory
发表于 2025-3-27 17:40:59
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insolence
发表于 2025-3-27 18:29:18
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fender
发表于 2025-3-28 01:08:48
A/C Simultaneous Conversion Using the Dual Base Editor in Human Cellseditors can only edit either adenines or cytosines. Thus, our lab has developed a dual base editor (A&C-BEmax) through the fusion of cytidine and adenosine deaminases to Cas9n to achieve both C•G to T•A and A•T to G•C mutations, which enables A/C simultaneous conversion in the same allele (up to 30%
载货清单
发表于 2025-3-28 04:40:30
Functional Analysis of Variants in BRCA1 Using CRISPR Base Editorsed to evaluate the function of the variants of uncertain significance (VUS) of the BRCA genes. However, these methods have limitations as they are associated with overexpression and do not apply to post-transcriptional regulation. Therefore, there are several VUS whose functions are unclear. Recentl
CAGE
发表于 2025-3-28 09:32:01
Use of the Representative Base Editing Tool Target-AID to Introduce Pathogenic Mutations into Micestranded DNA breaks. Target-AID (activation-induced cytidine deaminase) is a representative base editing tool and may serve as a potent option to create genetically modified animals that harbor disease-causing pathogenic point mutations. In this chapter, I describe the basic protocol used to introdu
BADGE
发表于 2025-3-28 14:19:18
Targeted Mutagenesis in Mice Using a Base Editored Short Palindromic Repeats (CRISPR)-based enzymatic tools for specific nucleotide substitutions. They are mainly the most effective genome editing tools for introducing point mutations, such as C-to-T and A-to-G conversions. The enhanced base editor, a C-to-G base editor (CGBE), can perform other