脆弱带来
发表于 2025-3-23 12:02:54
https://doi.org/10.1007/978-3-8348-9245-4e sequence and phosphorylation status of the 5′ ends of RNAs. 5′ specific RNA-seq can be used to analyze transcription initiation and posttranscriptional processing of RNAs with single base pair resolution on a genome-wide level.
侵害
发表于 2025-3-23 16:42:38
Wilfried Plaßmann,Detlef Schulzcetaldehyde for in situ footprinting of . elongation complex temporarily halted by a protein roadblock. The method provides valuable information on the dynamic features of transcriptionally engaged RNA polymerase within the cellular environment.
Custodian
发表于 2025-3-23 18:21:56
https://doi.org/10.1007/978-3-8348-9245-4nding assays from series used to determine the urea dependence of open complex formation and dissociation with . RNA polymerase and phage λP. promoter DNA. Then, we describe the subsequent data analysis and interpretation of these solute effects.
冷峻
发表于 2025-3-23 22:18:20
https://doi.org/10.1007/978-3-8348-9245-4(1) single-round transcription, (2) walking of RNAP to any defined template position, and (3) discrimination of transcripts that are associated with RNAP from those that are released to solution. This methodology is based on untagged proteins transcribing biotin- and digoxigenin-labeled DNA templates in association with paramagnetic particles.
废墟
发表于 2025-3-24 03:15:59
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Invigorate
发表于 2025-3-24 07:13:13
Differential- und Integralrechnungstrand designs where translocation of RNA polymerase from a pre-translocation to a post-translocation state results in disruption of stacking interactions of fluorophore with neighboring bases, with a concomitant large increase in fluorescence intensity.
使绝缘
发表于 2025-3-24 13:49:40
https://doi.org/10.1007/978-3-8348-9245-4through its stepwise translocation towards RNA polymerase. This system can be used to study the effects of concurrent translation on RNA chain elongation and to elucidate the interface between the two macromolecular complexes.
水汽
发表于 2025-3-24 16:39:36
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多山
发表于 2025-3-24 19:34:08
https://doi.org/10.1007/978-3-8348-9245-4 easily expressed and purified from heterologous expression systems. Forming functional RNA polymerase involves simply combining the different subunits under denaturing conditions and slowly removing the denaturant.
我没有命令
发表于 2025-3-25 01:27:19
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