极为愤怒
发表于 2025-3-28 16:23:14
Cell Fractionation,ent compartments: the cytoplasm, the inner membrane, the periplasm, the outer membrane, and the extracellular medium. Different approaches can be used to determine the protein localization within cell such as in silico identification of protein signal sequences and motifs, electron microscopy and im
空洞
发表于 2025-3-28 21:16:58
Components Subcellular Localization: Identification of Lipoproteins Using Globomycin and Radioactiva posttranslational modification in the cytoplasmic membrane. Here, I describe the detection of one of the intermediate forms of lipoprotein, diacylglyceryl-prolipoprotein, using .H-palmitate labeling and inhibition of signal peptidase II (Lsp) by globomycin and detection by fluorography.
样式
发表于 2025-3-29 00:24:11
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朝圣者
发表于 2025-3-29 06:50:30
Defining Membrane Protein Localization by Isopycnic Density Gradients,ranes. Classic techniques in cell biology can be applied to characterize bacterial membranes and their membrane protein constituents, and here we describe a protocol for the purification of outer membranes and inner membranes from .. This allows for compositional analysis of the membranes as well as
Mangle
发表于 2025-3-29 09:43:51
Components Subcellular Localization: Cell Surface Exposure,tional methods to predict surface-exposed lipoproteins, and therefore lipoprotein topology must be experimentally tested. This chapter describes several distinct but complementary methods for detection of surface-exposed proteins: cell surface protein labeling, accessibility to extracellular proteas
冥想后
发表于 2025-3-29 14:35:42
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mucous-membrane
发表于 2025-3-29 17:34:55
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intention
发表于 2025-3-29 22:33:38
Preparation of Uniformly Oriented Inverted Inner (Cytoplasmic) Membrane Vesicles from Gram-Negativeect translocation of lipids and proteins synthesized on cytoplasm facing leaflet of cytoplasmic (inner) membrane (IM), across IM and between IM and outer membrane (OM). Uniformly oriented inside-out (ISO) vesicles became functional requisite for many biochemical reconstitution functional assays, vec
PHON
发表于 2025-3-30 02:11:16
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macular-edema
发表于 2025-3-30 06:36:23
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