microscopic
发表于 2025-3-23 12:46:51
An Integrated Cell-Free Assay to Study Translation Regulation by Small Bacterial Noncoding RNAsm, motility, and pathogenesis. Using the bacterial carbon storage regulator/regulator of secondary metabolism (Csr/Rsm) system, we here describe an .-based cell-free translation assay that allows a quantitative analysis of translation regulation by ncRNAs and their corresponding translation represso
colony
发表于 2025-3-23 17:22:52
Quantitative Super-Resolution Imaging of Small RNAs in Bacterial Cellson (smFISH), super-resolved single-fluorophore microscopy, and clustering analysis. Compared to smFISH imaging with diffraction-limited fluorescence microscopy, our method provides better quantification for short and abundant RNA (such as sRNAs) in a small volume of bacterial cells. Our method can a
刺激
发表于 2025-3-23 21:22:20
http://reply.papertrans.cn/19/1804/180319/180319_13.png
得罪人
发表于 2025-3-24 01:22:31
http://reply.papertrans.cn/19/1804/180319/180319_14.png
callous
发表于 2025-3-24 03:37:04
High-Resolution, High-Throughput Analysis of Hfq-Binding Sites Using UV Crosslinking and Analysis ofcteria. They play key roles in most aspects of bacterial physiology, including central metabolism, nutrient acquisition, virulence, biofilm formation, and outer membrane composition. RNA sequencing technologies have accelerated the identification of bacterial regulatory RNAs and are now being employ
Noisome
发表于 2025-3-24 07:47:18
Identification of New Bacterial Small RNA Targets Using MS2 Affinity Purification Coupled to RNA SeqsRNAs expressed in vivo. After purification of the tagged-sRNA, we use RNAseq to determine the identity of all RNA interacting partners and their enrichment level. We describe how to analyze the RNAseq data through the Galaxy Project Platform bioinformatics tools to identify new mRNA targets. This technique is applicable to most sRNAs of . and ..
珊瑚
发表于 2025-3-24 13:53:34
Mapping Changes in Cell Surface Protein Expression Through Selective Labeling of Live Cellsthod comprises a direct labeling of surface proteins on living cells using fluorescent dyes, followed by total protein extraction and subsequent separation of these extracts by 2D gel electrophoresis.
无底
发表于 2025-3-24 15:04:40
http://reply.papertrans.cn/19/1804/180319/180319_18.png
APNEA
发表于 2025-3-24 21:18:48
http://reply.papertrans.cn/19/1804/180319/180319_19.png
动机
发表于 2025-3-25 02:21:19
Absolute Regulatory Small Noncoding RNA Concentration and Decay Rates Measurements in ,us our analyses on the . Gram-negative bacterium as a model. The information described in this chapter provides a guideline to help develop a protocol in order to assess these important parameters and to identify RNA-processing enzymes involved in sRNA degradation processes.